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TIBCO
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Image Search Results
Journal: The FASEB Journal
Article Title: Extracellular vesicles extracted from young donor serum attenuate inflammaging via partially rejuvenating aged T-cell immunotolerance
doi: 10.1096/fj.201800059R
Figure Lengend Snippet: Characteristics of serum-extracted EVs. A) Comparison of the sizes of EVs extracted from young murine serum by the ExoQuick reagent pretreated with and without using 0.2-μm filters. B) Morphology of EVs from young murine serum used in this project for rejuvenation of inflammaging, photographed by atomic force microscopy (AFM). C, D) Different miRNA expression profiles in heatmap (C) and quantified summary (D) of EVs from young vs. old murine serum, analyzed by murine miRNA microarray with Mus musculus miRBase version-21 arrays that contained 1900 unique mature miRNA probes (miRNA microarray service via LC Sciences).
Article Snippet: C , D ) Different miRNA expression profiles in heatmap ( C ) and quantified summary ( D ) of EVs from young vs. old murine serum, analyzed by
Techniques: Comparison, Microscopy, Expressing, Microarray
Journal: Journal of Cardiovascular Development and Disease
Article Title: The CCR2 + Monocyte Subsets Increase in Obese Boys but Not Girls with Abnormally High Carotid Intima-Media Thickness: A Pilot Study
doi: 10.3390/jcdd9100330
Figure Lengend Snippet: Gating Strategy. We first gated total leukocytes on a time/side scatter density plot and selected the Zombie UV negative cell population for detecting living cells. Next, we gated living cells for singlets on a forward scatter/trigger pulse width density plot. After recognizing cells by size and granularity, we selected monocytes on the HLA-DR gate. Then, we gated monocytes using the rectangular gating strategy on the CD14 + /CD16 + cell population to identify CD14 ++ CD16 − cells as classical monocytes, CD14 ++ CD16 + cells as intermediate monocytes, and CD14 + CD16 + cells as nonclassical monocytes . We obtained the median fluorescence intensity (MFI) for CCR2 by considering both positive and negative cell populations. We got the CCR2 + cell percentage using fluorescence minus one (FMO) control. For each fluorochrome, we used compensation controls through UltraComp eBeads TM (Invitrogen TM , Carlsbad, CA, USA). We analyzed data by the FlowJo 10.0.7 software (TreeStar, Inc., Ashland, OR, USA).
Article Snippet: We analyzed monocyte subpopulations using the
Techniques: Fluorescence, Software